Which enzyme will be produced in a cell in which there is a non-sense mutation in the lac Y gene ?
- A.
Transacetylase
- B.
Lactose permease and transacetylase
- C.
Lactose permease
- D.
-galactosidase
- Recall the gene order in the lac operon.
Given that the structural genes of the lac operon are arranged in the order , , , coding respectively for beta-galactosidase, permease, and transacetylase, transcription and translation proceed sequentially along this order.
- Understand the effect of a nonsense mutation.
Given that a nonsense mutation introduces a premature stop codon, translation halts at that point, meaning any genes coming after the mutated gene in the sequence also fail to be expressed properly.
- Apply this to a mutation in the gene.
Since the mutation occurs in the gene, translation stops there, preventing the production of permease and the downstream transacetylase ( gene).
- Determine what is still produced.
Given that the gene comes before the gene in the sequence, its translation is unaffected, so beta-galactosidase is still produced normally.
Conclusion: Hence, the answer is D, -galactosidase.