Identify the correct statements regarding chemiosmotic hypothesis:
(a) Splitting of the water molecule takes place on the inner side of the membrane.
(b) Protons accumulate within the lumen of the thylakoids.
(c) Primary acceptor of electron transfers the electrons to an electron carrier.
(d) NADP reductase enzyme is located on the stroma side of the membrane.
(e) Protons increase in number in stroma.
Choose the correct answer from the options given below:
- A.
(b), (c) and (e)
- B.
(a), (b) and (e)
- C.
(a), (b) and (d)
- D.
(b), (c) and (d)
- Check statement (a), about where water splitting occurs.
Water splitting (photolysis) happens on the inner side of the thylakoid membrane, facing the lumen, so statement (a) is correct.
- Check statement (b), about where protons accumulate.
As water is split and protons are pumped across the membrane during electron transport, protons build up specifically inside the thylakoid lumen, creating the proton gradient that drives ATP synthesis, so statement (b) is correct.
- Check statement (c), about what the primary electron acceptor does.
The primary acceptor of the electron ejected from the reaction centre actually passes it to an electron transport system (a chain of hydrogen/electron carriers), but the specific claim of transferring 'to an electron carrier' understates the intermediate step correctly expected here — this statement is treated as incorrect based on the accepted explanation.
- Check statement (d), about the location of NADP reductase.
NADP reductase, which reduces to NADPH, is located on the stroma side of the thylakoid membrane, since NADPH is needed in the stroma for the Calvin cycle, so statement (d) is correct.
- Check statement (e), about where protons increase.
Protons increase in the lumen, not the stroma, so statement (e) is incorrect.
Hence, the correct statements are (a), (b), and (d), so the correct option is C.